Fig. 5.
![Fig. 5. Effect of the antagonist anti-Fas MoAb ZB4 on HLA class I–induced apoptosis. PBL were activated with PHA (5 μg/mL) for 3 days. Viable cells were pre-incubated with the antagonist anti-Fas MoAb ZB4 for 1 hour and then treated with medium, YTH862 (10 μg/mL), MoAb90 (10 μg/mL), or the agonist anti-Fas MoAb CH11 (1 μg/mL) for 20 hours. The percentage of apoptotic cells was determined by fluorescent microscopy after staining with Hoescht 33342. In parallel, inhibition of [3H]TdR uptake during the last 8 hours of culture was measured. Results are expressed as mean ± SD of four independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3629/4/bl_0035f5.jpeg?Expires=1735823999&Signature=1HfZ00Ni4vjsxo3hd9N0kdo~pkYgX~C9F4XUPFa11xmXdGNNuhYevzGVaxPn4t1ffqzldypwQihaYEYsb3hSCETZ95s06euqInw0MImgQSHzZe~xhNhz--hg6Iwhv9Uc2BMRjj-wbrQsSH17dr4UKNZK1R36T9dVGlFWiGQLyLbRkWTjEB6J9YW7LEIlJWuV~y1ukDvNm-~SwWSNl9NUC-~aQY~1IDnLtvd8O~N~Yg3IFAYNsE9Bb9TJnL-qphZjVYUIGomFzv-0VvowYzzlvKRWeiN1ZC-OKl-A3J4wVXx-RdlqxWc9oovHahH6KjBHn3TJS2lHBQ5FDfUmRMmDWw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the antagonist anti-Fas MoAb ZB4 on HLA class I–induced apoptosis. PBL were activated with PHA (5 μg/mL) for 3 days. Viable cells were pre-incubated with the antagonist anti-Fas MoAb ZB4 for 1 hour and then treated with medium, YTH862 (10 μg/mL), MoAb90 (10 μg/mL), or the agonist anti-Fas MoAb CH11 (1 μg/mL) for 20 hours. The percentage of apoptotic cells was determined by fluorescent microscopy after staining with Hoescht 33342. In parallel, inhibition of [3H]TdR uptake during the last 8 hours of culture was measured. Results are expressed as mean ± SD of four independent experiments.
