Fig. 7.
PBL were activated with PHA (5 μg/mL) for 3 days. Viable cells were then treated by antibodies MoAb90 (10 μg/mL), YTH862 (10 μg/mL), or anti-Fas MoAb CH11 (1 μg/mL) alone or in association and measurements of apoptosis were performed as in Fig 6. In parallel, CD45RA and CD45RO phenotype after 3 days of culture was evaluated by FACS analysis (CD45RA+ 22 [2.4%], CD4RO+ 32 [0.5%], CD45RA+RO+ 32 [0.5%]) (SEM in brackets).