Fig. 5.
Effect of anti–TNF-α-MoAb on LPS-induced suppression of neutrophil apoptosis. Freshly isolated neutrophils (1 × 106/mL) from healthy humans were cultured with LPS (1 μg/mL) in the presence or absence of neutralizing anti–TNF-α-antibodies (10 μg/mL) during 16 hours. Neutrophil apoptosis was analyzed by flow cytometry after permeabilization with ethanol and staining with propidium iodide. Data are reported as the percentage of fragmented nuclei reflecting the relative proportion of apoptotic cells. Results represent the mean ± SEM of three separate experiments performed with neutrophils isolated from three different healthy individuals. *P < .05 without agent versus LPS; ‡P < .05 without anti–TNF-α-MoAb versus with anti–TNF-α-MoAb.
