Effect of SLF or IL-3 with Ca2+ influx blockers on 32D-Kit cells. (A through D) 32D-Kit cells were incubated with either SLF (500 ng/mL in all cases) (circles) or IL-3 (25% WEHI-3 conditioned medium in all cases) (squares) or both SLF and IL-3 (triangles) with varying amounts of econazole or ketotifen. In the case of (A) and (C), the proportion of dead cells was determined after 18 hours in culture by counting cells that could or could not exclude Trypan blue. For (B) and (D), colony formation was determined by incubating cells in liquid culture with either SLF or IL-3 with econazole or ketotifen followed by 7 days of incubation of cells in semisolid medium. (E) 32D-Kit cells were incubated with varying amounts of SLF. (Circles) Plus 7.5 μmol/L econazole. (Squares) No econazole. (F) 32D-Kit cells were incubated with varying amounts of SLF for 36 hours followed by a 6-hour pulse with 3HdT thymidine, harvesting, and counting. Error bars represent the standard error determined from triplicate measurements.
