Effect of SLF or IL-3 with Ca²⁺ influx blockers on BMMC. (A through C) BMMC were incubated with either SLF (500 ng/mL in all cases) (circles) or IL-3 (25% WEHI-3 conditioned medium in all cases) (squares) or both SLF and IL-3 (triangles) with varying amounts of econazole, ketotifen, or Ni²⁺. (D) BMMC were incubated with IL-3 and varying amounts of SLF. (Circles) Plus 2 mmol/L Ni²⁺. (Squares) No Ni²⁺. In all cases, the proportion of dead cells was determined after 18 hours in culture by counting cells that could or could not exclude Trypan blue. Error bars represent the standard error determined from triplicate measurements.