Western blot analysis of nuclear PARP. 32D-Kit cells were incubated for 12 hours with either SLF (lane 1), IL-3 (lane 2), SLF plus 10 μmol/L econazole (lane 3), IL-3 plus 10 μmol/L econazole (lane 4), or SLF plus 10 μmol/L econazole plus 100 μmol/L Boc-Asp-FMK. Nuclear lysates from these cultures were separated by 7.5% SDS-PAGE, transferred to nitrocellulose, and probed with anti-PARP antibodies.