Influence of hematopoietic cytokines on progenitor cell migration and ³H thymidine incorporation. Ten thousand FDCP-mix cells (▪) or 5,000 lin⁻Sca1⁺ cells (□) were seeded into Fn-coated 96 wells and migration as well as cell proliferation were determined after 24 hours in tilted position as described in Materials and Methods. Cytokine concentrations used were: EPO, 2 U/mL; M-CSF, 50 U/mL; G-CSF, 30 ng/mL; GM-CSF, 50 ng/mL; IL-3, 100 U/mL; IL-1α, 5 ng/mL; IL-6, 10 ng/mL; SCF, 50 ng/mL; TNF-α, 10 ng/mL; TGF-β, 10 ng/mL; M-CAF, 10 ng/mL; MIP-1 α, 10 ng/mL; IL-8, 20 ng/mL. Shown are means ± SD from three independent cultures. * Indicates statistically significant difference from controls (P < .05, Student's t-test).