Video image analysis of migrating cells. Ten thousand FDCP-mix cells were seeded into 96-well migration assays precoated with 10 μg/cm2 Fn in the presence of 10 U/mL IL-3, and analyzed at a 15°C tilted position for a 20-minute period using time lapse video microscopy. Three representative progenitor cells, each imaged at different time points within an identical section of the observed field, are shown at 1,000× orginal magnification. Values indicated are time points in minutes:seconds after the start of the observation period.