PCR-SSP of D^VI samples. Agarose gels of representative D^VI samples are shown along with negative and positive controls. The specificities of the 12 reactions for the RHD/RHCE exons are shown on the right side (box). The nucleotide position(s) detected by the PCR-SSP are given along with the expected sizes of the specific products. The control band represents a 434-bp product of the growth hormone gene. For D^VI samples, three different reaction patterns are observed: pattern I (B) lacks specific signals for RHD exons 4 and 5 and is compatible with D^VItype I.19 Pattern II (C) lacks those for RHD exons 4 to 6 being compatible with D^VItype II.18 Pattern III (D) lacking specific products forRHD exons 3 to 6 is novel. c² indicates the c(cyt48) allele.