Fig. 2.
Erk activity of CMK stable transfectants. (A) Western blot analysis of individual clones of transfected CMK cells using an anti-Phospho–MAPK antibody. (B) Erk activity (mean ± standard error of mean [SEM]) of lysates from three to nine separate CMK clones transfected with vector, WT Mek, DE Mek, or D330N Erk as indicated. The mean Erk activity in DE Mek and D330N Erk transfected cells was significantly greater than that found in vector and WT Mek transfected cells (* P < .05, ** P < .01 by ANOVA).