Fig. 1.
The −245 hPF4/lacZ DNA construct used in the creation of transgenic mice. Primers used in PCR screening (mss3 and β2), semiquantitative RT-PCR (β5 and β2), and the probe for Southern blots (3.5 kb BamHI fragment) are indicated with approximate locations of a spliceable intron (int) and polyadenylation signal (pA) sequences.