Fig. 2.
Transgene detection by Southern blot analysis of two transgenic mouse lines. Lanes 1, 2, and 3 contain 30, 60, and 120 pg of transgene construct DNA, BamHI-digested. Lanes 4 and 7 are blank. Lane 5 contains 10 μg of BamHI-digested genomic DNA from F0 no. 3, and lane 6 F0 no. 16. Lane 8 contains 10 μg of BamHI-digested genomic DNA from a negative littermate control. The blot was hybridized with the labeled lacZ probe shown in Fig 1. The expected 3.5-kb band for the transgene is denoted by the arrow. Hybridization with an endogenous mouse gene showed the expected band for lanes 5, 6, and 8 (data not shown).
