Level of human cell engraftment in the BM of NOD/SCID mice transplanted with chronic phase CML cells. (A) Southern blot in which each lane contains DNA from a single mouse transplanted with 108 light density cells from the BM or PB from CML patient 2. Some of the mice subsequently received intraperitoneal injections of human MGF (M), PIXY321 (P), GM-CSF (GM), and G-CSF (G) on alternate days (no GF = no growth factors). Mice were killed between 3 and 29 weeks posttransplant, or earlier if they appeared sick (arrows). Mice killed after 5 months are indicated by an asterisk. DNA extracted from cells obtained from the BM or spleen (S) of the mice was digested withEcoRI and probed to detect a human-specific a-satellite sequence (p17H8).39 The level of human cell engraftment was determined by comparing the intensity of the 2.7-kb band with those of defined mixtures of human and mouse DNA as shown. (B) Level of engraftment in the BM of 65 mice transplanted with BM (• and ▴) or PB (○ and ▵) cells from the 11 patients with chronic phase CML studied, as determined by Southern blot analysis (• and ○) or flow cytometry (▴ and ▵) using antibodies to CD45 and CD71. The number of cells transplanted per mouse ranged from 7.5 × 107 to 108 except for two mice that received 2 × 107PB cells and two mice that received 8 × 106 PB cells. (—) Indicate geometric means.
