Fig. 4.
Transplantation of CD34+ cell-enriched chronic phase CML cells into NOD/SCID mice. (A) Southern blot showing the level of human cell engraftment in the BM of mice transplanted with 2 × 105 (lanes 4 and 5) or 2 × 106 (lanes 1 through 3 and 6 and 7) CD34+ cells (purity 63%) isolated from the PB of a patient with chronic phase CML. Mice were analyzed after 5 to 7 weeks except for mouse 3 and mouse 6 which were analyzed after only 2 to 3 weeks because of morbidity. DNA analysis was performed as described in Fig 1A. (B) BCR Southern analysis of the BM of mice 1 through 3 from (A). For methods, refer to Fig 3.
