Fig. 3.
![Fig. 3. Maturation of dendritic cells induced by MCM is irreversible. GM-CSF/IL-4–treated ER− cells were cultured for 4 days in medium alone [−MCM], two different MCM preparations (MCM A and B), or a combination of cytokines equal to that present in the MCM. On day 11, the cells were washed and analyzed for CD83 expression. Residual cells were returned to culture for 3 more days (day 14) in the absence of further supplementation and analyzed for the retention of CD83. MCM contained the following cytokines: (A) IL-1 (92 ng/mL), IL-6 (1 μg/mL), IFN-α (0.02 ng/mL), and TNF-α (182 ng/mL); (B) IL-1 (20 ng/mL), IL-6 (734 ng/mL), IFN-α (.08 ng/mL), and TNF-α (24 ng/mL).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3640/4/bl_0044f3.jpeg?Expires=1736046061&Signature=Ko0pqQdKJ-G77uGVCSGF7JmjfMxpTDLWgostWhV1VYO1HEfl9ljVxmOQl6WlChEayup-LNyEkeP18ckhictwYMRjYQeOZnqb5Mb-8a8fntcXa9tDrmGwT4S5ach4W0OWI3WhHjt4n5d31qV-cMqDq5UYAT7io4CYtspUlZ9U6hMvaSbieH0Kw3tbl5f1QKuYfGI1vWhg5-502KdRGNXIhrsYa2KA04G8PyV4V7o24~dNXEjg17dPkAQEhV3iYTF1Zg83CFHLNcEhMxKuCj8aur0~xGwwBZRFqdWNTqESaHkIQ2ndjizMtgAGhBpIuLECUudWRHTezNnoONkSFLsnUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Maturation of dendritic cells induced by MCM is irreversible. GM-CSF/IL-4–treated ER− cells were cultured for 4 days in medium alone [−MCM], two different MCM preparations (MCM A and B), or a combination of cytokines equal to that present in the MCM. On day 11, the cells were washed and analyzed for CD83 expression. Residual cells were returned to culture for 3 more days (day 14) in the absence of further supplementation and analyzed for the retention of CD83. MCM contained the following cytokines: (A) IL-1 (92 ng/mL), IL-6 (1 μg/mL), IFN-α (0.02 ng/mL), and TNF-α (182 ng/mL); (B) IL-1 (20 ng/mL), IL-6 (734 ng/mL), IFN-α (.08 ng/mL), and TNF-α (24 ng/mL).
