Fig. 2.
ATGs induce apoptosis of activated T lymphocytes. PBL were cultured in presence of medium alone or PHA (5 μg/mL) for 3 days. Dead cells were removed and viable cells were treated for 20 hours with ATG no. 95-07, F(ab)′2 fragments of ATG no. 95-07, ATG no. 5 or normal rabbit IgG at 10 μg/mL or with the agonist anti-Fas MoAb CH11 at 1 μg/mL. Protection by the antagonist anti-Fas MoAb, was tested by pre-incubating PBL or PHA-activated cells for 1 hour with ZB4 MoAb at 2 μg/mL. The percentage of apoptotic cells was determined by fluorescent microscopy after staining with Hoechst 33342. Results are expressed as mean ± SEM of five different experiments or as mean of two experiments for ATG no. 5.
