Fig. 4.
![Fig. 4. Effect of ATGs on CD69, CD25, Fas, and Fas-L surface expression. PBL were cultured in presence of medium alone or ATG no. 95-07 and ATG no. 5 at 10 μg/mL and 100 μg/mL for 3 days. At days 0, 1, 2, and 3, surface expression of CD69, CD25, Fas, and Fas-L was determined by cytofluorometry. In parallel, incorporation of [3H]TdR uptake during the last 8 hours of culture was measured (med 367 ± 41 cpm, ATG no. 5 10 μg/mL 391 ± 23 cpm, ATG no. 5 100 μg/mL 252 ± 26 cpm, ATG no. 95-07 10 μg/mL 532 ± 53 cpm, and ATG no. 95-07 100 μg/mL 11,500 ± 103 cpm). Histograms of Fas-L expression at day 1 are shown. Representative of four experiments with ATG no. 95-07 and of two with ATG no. 5.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2360/3/blod4071904.jpeg?Expires=1735832459&Signature=Eebi6CLWfijzphY1~SfvcxKSVwqBJ5DbQQYDqgiTVP7SuHbpinWQ1THLxoypSOHOuGpgiIz0-ztf51L4VR~XeimZOwJPb6fnyQEwq0rXFP2SPycjJygKPNIKGk-ualy~H15oIlD-4K62F7~nlNfZA0aOwRlHJEcMeXj32AWlN7YutfD7uNX6P2~xX9KAz6dc5WJy6mv0GV-~tO0UvdqGbQL5o2beYSlDHqjW~XHLyF22og3UlwXcKXm7YWl-bqpxPU8JKqCDJ-ZqpDusU4R~aGkfBCyEDMFnDwmlMH58xzs5BzMaoqW5Cir4PI9Bm5qlpPS2eaV8KERQImDfVvtG-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of ATGs on CD69, CD25, Fas, and Fas-L surface expression. PBL were cultured in presence of medium alone or ATG no. 95-07 and ATG no. 5 at 10 μg/mL and 100 μg/mL for 3 days. At days 0, 1, 2, and 3, surface expression of CD69, CD25, Fas, and Fas-L was determined by cytofluorometry. In parallel, incorporation of [3H]TdR uptake during the last 8 hours of culture was measured (med 367 ± 41 cpm, ATG no. 5 10 μg/mL 391 ± 23 cpm, ATG no. 5 100 μg/mL 252 ± 26 cpm, ATG no. 95-07 10 μg/mL 532 ± 53 cpm, and ATG no. 95-07 100 μg/mL 11,500 ± 103 cpm). Histograms of Fas-L expression at day 1 are shown. Representative of four experiments with ATG no. 95-07 and of two with ATG no. 5.
