Human neutrophils and monocytes take up dehydroascorbic acid through facilitative glucose transporters. (A) Uptake of deoxyglucose (DOG) by human neutrophils (○) and monocytes (•). (B) Transport of methylglucose (OMG) by human neutrophils (○) and monocytes (•). (C) Effect of competitors and inhibitors on the uptake of dehydroascorbic acid by human neutrophils. Uptake was assayed in the absence (control) or in the presence of 30 mmol/L deoxyglucose, methylglucose, or L-glucose (LG), or the cells were incubated with 20 μmol/L cytochalasin B (CytB) or cytochalasin E (CytE) for 5 minutes before the uptake assay. (D) Effect of competitors and inhibitors on the uptake of dehydroascorbic acid by human monocytes. Uptake was assayed in the absence (control) or in the presence of 30 mmol/L deoxyglucose, methylglucose, or L-glucose, or the cells were incubated with 20 μmol/L cytochalasin B or cytochalasin E for 5 minutes before the uptake assay. For (A and B), the data represent the mean ± SD of four samples and correspond to one of three similar experiments. For (C and D), the data represent the average of two experiments performed in duplicate.