Effect of GM-CSF on the uptake of deoxyglucose (DOG) and methylglucose (OMG) by HL-60 neutrophils. (A) Double reciprocal plot of the effect of GM-CSF on the substrate dependence for deoxyglucose transport. Cells were incubated for 30 minutes in the absence (○) or in the presence (•) of 0.5 nmol/L GM-CSF and transport of deoxyglucose was measured at 30 seconds. Data represent the mean of four samples. (B) Double reciprocal plot of the effect of GM-CSF on the substrate dependence for methylglucose transport. Cells were incubated for 30 minutes in the absence (○) or in the presence (•) of 0.5 nmol/L GM-CSF and transport of methylglucose was measured at 30 seconds. Data represent the mean of four samples and correspond to one of three similar experiments.