Fig. 7.
Fig. 7. Effect of fMetLeuPhe on the uptake of dehydroascorbic acid (DHA) and ascorbic acid (AA) by HL-60 neutrophils. (A) Effect of GM-CSF and fMetLeuPhe on the uptake of ascorbic acid (AA). Cells were incubated in the absence (○, ▪) or in the presence (▴) of GM-CSF for 30 minutes. Afterwards, uptake of ascorbic acid was assayed in the absence (control, ○) or in the presence (▪, ▴) of 5 μmol/L fMetLeuPhe. (B) Effect of fMetLeuPhe on the initial uptake of ascorbic acid. Uptake of ascorbic acid was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (C) Effect of superoxide dismutase and catalase on the uptake of ascorbic acid uptake. Uptake was measured for 10 minutes in the presence of fMetLeuPhe and superoxide dismutase (SOD) or catalase. (D) Effect of deoxyglucose (DOG) on the uptake of ascorbic acid in cells treated with fMetLeuPhe. Uptake of ascorbic acid was assayed in the absence (○) or in the presence (□, •) of 5 μmol/L fMetLeuPhe and 50 mmol/L deoxyglucose (□). (E) Effect of deoxyglucose on the initial phase of transport of ascorbic acid (AA) by cells treated with fMetLeuPhe. Cells were incubated in medium containing radiolabeled ascorbic acid and 5 μmol/L fMetLeuPhe with (○) or without (•) 50 mmol/L deoxyglucose. (F) Effect of fMetLeuPhe on the transport of dehydroascorbic acid. Transport was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (G) Effect of fMetLeuPhe on the long-term uptake of dehydroascorbic acid. Uptake was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (H) Effect of fMetLeuPhe on transport of deoxyglucose. Transport was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (I) Effect of fMetLeuPhe on the long-term uptake of deoxyglucose. Uptake was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (I) Effect of fMetLeuPhe on the transport of methylglucose (OMG). Transport was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. Data represent the mean ± SD of four samples and correspond to one of two to four similar experiments.

Effect of fMetLeuPhe on the uptake of dehydroascorbic acid (DHA) and ascorbic acid (AA) by HL-60 neutrophils. (A) Effect of GM-CSF and fMetLeuPhe on the uptake of ascorbic acid (AA). Cells were incubated in the absence (○, ▪) or in the presence (▴) of GM-CSF for 30 minutes. Afterwards, uptake of ascorbic acid was assayed in the absence (control, ○) or in the presence (▪, ▴) of 5 μmol/L fMetLeuPhe. (B) Effect of fMetLeuPhe on the initial uptake of ascorbic acid. Uptake of ascorbic acid was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (C) Effect of superoxide dismutase and catalase on the uptake of ascorbic acid uptake. Uptake was measured for 10 minutes in the presence of fMetLeuPhe and superoxide dismutase (SOD) or catalase. (D) Effect of deoxyglucose (DOG) on the uptake of ascorbic acid in cells treated with fMetLeuPhe. Uptake of ascorbic acid was assayed in the absence (○) or in the presence (□, •) of 5 μmol/L fMetLeuPhe and 50 mmol/L deoxyglucose (□). (E) Effect of deoxyglucose on the initial phase of transport of ascorbic acid (AA) by cells treated with fMetLeuPhe. Cells were incubated in medium containing radiolabeled ascorbic acid and 5 μmol/L fMetLeuPhe with (○) or without (•) 50 mmol/L deoxyglucose. (F) Effect of fMetLeuPhe on the transport of dehydroascorbic acid. Transport was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (G) Effect of fMetLeuPhe on the long-term uptake of dehydroascorbic acid. Uptake was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (H) Effect of fMetLeuPhe on transport of deoxyglucose. Transport was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (I) Effect of fMetLeuPhe on the long-term uptake of deoxyglucose. Uptake was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. (I) Effect of fMetLeuPhe on the transport of methylglucose (OMG). Transport was assayed in the absence (○) or in the presence (•) of 5 μmol/L fMetLeuPhe. Data represent the mean ± SD of four samples and correspond to one of two to four similar experiments.

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