American Society of Hematology

Fig. 3.

$Fig. 3. Eosinophil release from bone marrow induced by eotaxin. The femoral bone marrow was perfused in situ with modified Krebs-Ringer bicarbonate buffer via the external iliac artery and 10-minute fractions were collected from the external iliac vein. (A) Kinetics of eosinophil release after a 10-minute infusion (indicated by ▩) of eotaxin (3 nmol/L) or vehicle (PBS/0.1% BSA). Results represent the number of eosinophils per milliliter of perfusate in each 10-minute fraction, mean ± SEM (n = five to six perfusions). (•), Eotaxin 3 nmol/L; (○), Vehicle. (B) Total eosinophil release and total release of other leukocytes induced by a 10-minute infusion of eotaxin (0.03 and 3 nmol/L) or vehicle. Results show the total number of eosinophils or other leukocytes released during the 80-minute perfusion period, mean ± SEM (n = five to six perfusions). A significant difference between eotaxin and vehicle injected group is indicated by **(P < .01). (C) Kinetics of eosinophil release after a 30-minute infusion (indicated by ▩) of eotaxin (3 nmol/L) or vehicle. Results represent the number of eosinophils per milliliter of perfusate in each 10-minute fraction, mean ± SEM (n = four perfusions). (•) Eotaxin, 3 nmol/L; (○), Vehicle. (D) Eosinophil mobilization after a 30-minute infusion of eotaxin (3 nmol/L) or vehicle. Results (from experiment shown in C) show the total number of eosinophils released during the 110-minute perfusion period (left y-axis) and the number of eosinophils present in the femoral bone marrow after the 110-minute perfusion period (right y-axis), mean ± SEM (n = four perfusions). A significant difference between eotaxin and vehicle infused groups is indicated by *(P < .05) or **(P < .01).$

Eosinophil release from bone marrow induced by eotaxin. The femoral bone marrow was perfused in situ with modified Krebs-Ringer bicarbonate buffer via the external iliac artery and 10-minute fractions were collected from the external iliac vein. (A) Kinetics of eosinophil release after a 10-minute infusion (indicated by ▩) of eotaxin (3 nmol/L) or vehicle (PBS/0.1% BSA). Results represent the number of eosinophils per milliliter of perfusate in each 10-minute fraction, mean ± SEM (n = five to six perfusions). (•), Eotaxin 3 nmol/L; (○), Vehicle. (B) Total eosinophil release and total release of other leukocytes induced by a 10-minute infusion of eotaxin (0.03 and 3 nmol/L) or vehicle. Results show the total number of eosinophils or other leukocytes released during the 80-minute perfusion period, mean ± SEM (n = five to six perfusions). A significant difference between eotaxin and vehicle injected group is indicated by **(P < .01). (C) Kinetics of eosinophil release after a 30-minute infusion (indicated by ▩) of eotaxin (3 nmol/L) or vehicle. Results represent the number of eosinophils per milliliter of perfusate in each 10-minute fraction, mean ± SEM (n = four perfusions). (•) Eotaxin, 3 nmol/L; (○), Vehicle. (D) Eosinophil mobilization after a 30-minute infusion of eotaxin (3 nmol/L) or vehicle. Results (from experiment shown in C) show the total number of eosinophils released during the 110-minute perfusion period (left y-axis) and the number of eosinophils present in the femoral bone marrow after the 110-minute perfusion period (right y-axis), mean ± SEM (n = four perfusions). A significant difference between eotaxin and vehicle infused groups is indicated by *(P < .05) or **(P < .01).

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