American Society of Hematology

Fig. 6.

$Fig. 6. Eotaxin (EOT) and IL-5 act synergistically to release eosinophils from bone marrow. Perfusion of the femoral bone marrow was performed for a total of 110 minutes. (A) Kinetics of eosinophil release induced by a 10-minute infusion (indicated by ▩ 1) of IL-5 (0.3 nmol/L) or vehicle (PBS/0.1% BSA) followed 20 minutes later by a 10-minute infusion (indicated by ▩ 2) of eotaxin (0.3 nmol/L) or vehicle. Results represent the number of eosinophils per milliliter of perfusate in each 10-minute fraction, mean ± SEM (n = five to eight perfusions). A significant difference between IL-5 plus eotaxin and IL-5 alone at equivalent time point represented by **(P < .01) or ***(P < .001). (B) Total eosinophil release and total release of other leukocytes induced by a 10-minute infusion of IL-5 (0.3 nmol/L) or vehicle followed 20 minutes later by a 10-minute infusion of eotaxin (0.3 nmol/L) or vehicle. Results show the total number of eosinophils or other leukocytes released during the 110-minute perfusion period, mean ± SEM (n = five to eight perfusions). A significant difference between IL-5 alone and vehicle is indicated by †(P < .05) and a significant difference between IL-5 plus eotaxin and IL-5 alone by **(P < .01).$

Eotaxin (EOT) and IL-5 act synergistically to release eosinophils from bone marrow. Perfusion of the femoral bone marrow was performed for a total of 110 minutes. (A) Kinetics of eosinophil release induced by a 10-minute infusion (indicated by ▩ 1) of IL-5 (0.3 nmol/L) or vehicle (PBS/0.1% BSA) followed 20 minutes later by a 10-minute infusion (indicated by ▩ 2) of eotaxin (0.3 nmol/L) or vehicle. Results represent the number of eosinophils per milliliter of perfusate in each 10-minute fraction, mean ± SEM (n = five to eight perfusions). A significant difference between IL-5 plus eotaxin and IL-5 alone at equivalent time point represented by **(P < .01) or ***(P < .001). (B) Total eosinophil release and total release of other leukocytes induced by a 10-minute infusion of IL-5 (0.3 nmol/L) or vehicle followed 20 minutes later by a 10-minute infusion of eotaxin (0.3 nmol/L) or vehicle. Results show the total number of eosinophils or other leukocytes released during the 110-minute perfusion period, mean ± SEM (n = five to eight perfusions). A significant difference between IL-5 alone and vehicle is indicated by †(P < .05) and a significant difference between IL-5 plus eotaxin and IL-5 alone by **(P < .01).

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