Fig. 4.
Time kinetics of cytokine secreting cells/105PBMC in patient no. 2. PBMC were stimulated in vitro with F(ab′)2 fragments of the autologous monoclonal IgG (10 pg/mL). Number of cells secreting IFN-γ (▪), IL-2 (▴), and IL-4 (•) was assessed by ELISPOT. Number of cells secreting IFN-γ (□), IL-2 (▵), and IL-4 (○) induced by control monoclonal IgG. Arrows indicate immunization times.