TcR spectratyping complexity score sums over time. Complexity scores were generated from CDR3 spectratype analysis at various time points after transplantation. Individual complexity scores were generated for each Vβ TcR, which correlates to the number of peaks and their relative peak heights generated by autofluorescence of electrophoretic gels after RT-PCR of cDNA extracted from PBLs. Individual scores were summed to give an overall complexity score for that patient's peripheral TcR repertoire at that point in time. Open circles represent scores for the patients who received FACS-sorted CD34⁺ HPC transplants. Broken line represents the median values for all eight patients analyzed. Closed circles represent the control patient values. Shaded area and dashed line indicate the mean and standard deviation of seven normal healthy donors.