Effect of GAS6 soluble ligands on PMN adhesion to resting EC. Resting EC were treated or not for 10 minutes with either GAS6 (1 μg/mL) or Axl-x (1 μg/mL), and adhesion of PMN was then assessed. Treatment with Axl-x significantly potentiated PMN adhesion, whereas the control MoAb did not display any effect. Results are expressed as relative adhesion percent and represent the mean ± SD from five experiments. Asterisks mark values that are significantly different from the control (P < .05) and 100% relative adhesion (bold horizontal line) corresponds to an absolute adhesion of 11 ± 5 cells/microscope field.