Western blot analysis of fibrin induced IL-8 expression in HUVEC. Twenty-four hour control or fibrin treated HUVEC supernatants were either immunoprecipitated with anti-IL-8 antibody before being applied to SDS-PAGE or added to SDS-PAGE directly. The resulting blot was analyzed by standard Western blot assay. The molecular weight marker showed on lane 1. Human recombinant IL-8 (50 ng) as positive control was on lane 2. The control or fibrin treated HUVEC samples with anti-IL-8 antibody immunoprecipitation were on lane 3 and lane 4. The samples without immunoprecipitation were on lane 5 or lane 6, respectively. Lane 7 and lane 8 were samples from thrombin treated cells or fibrinogen itself (1 mg/mL), respectively.