American Society of Hematology

Fig. 6.

$Fig. 6. Eosinophils that were subfractionated to determine effects of IFNγ incubation (500 U/mL) on IL-6 concentration or localization. Quantification of IL-6 in each fraction was made by ELISA. Marker enzyme assays used in these experiments were EPO and β-hexosaminidase. The experiments were conducted at different times using purified blood eosinophils from the same donor. (A) Unstimulated eosinophils, followed by eosinophils stimulated for (B) 10 minutes and (C) 16 hours by IFNγ. An average of 50 × 106eosinophils were used for each preparation.$

Eosinophils that were subfractionated to determine effects of IFNγ incubation (500 U/mL) on IL-6 concentration or localization. Quantification of IL-6 in each fraction was made by ELISA. Marker enzyme assays used in these experiments were EPO and β-hexosaminidase. The experiments were conducted at different times using purified blood eosinophils from the same donor. (A) Unstimulated eosinophils, followed by eosinophils stimulated for (B) 10 minutes and (C) 16 hours by IFNγ. An average of 50 × 10⁶eosinophils were used for each preparation.

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