Fig. 5.
![Fig. 5. Coimmunoprecipitation of p27Kip1 and cdk2. 107 U937 cells were treated with PMA for 48 hours. p27Kip1 was immunoprecipitated (IP-1, IP-2, and IP-3) three times from the same extract. Both the immunoprecipitate and the supernatant either before (Pre-IP supernatant [sup]) or after the immunoprecipitate (Post-IP sup) were run on an SDS polyacrylamide gel and Western blotted with p27 antibody. The Western blot was then stripped and reprobed with cdk2 antibody.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3430/4/bl_0004f5.jpeg?Expires=1735561248&Signature=M6lTrC2KfHxJUPQi1TdPmLJOmpTPZIKP4-nd2Gwr1mQFDxabmXT2M6FxvCaQa6p4wPpYAVtY2ZbPvgtwvPmoJEU8uhu7g6~pM8OGEmpwHKjHz-SrnViZWEVJU24suSQN8Qxv1E9qmSWBvnryppEHNSEFaMgHTkw0cP5z-NuuXCZZd7RWKxRjN~v1UmDymoQLdFJEnQUAHJpHSS9CuZNeEcT8TrATeQhltyEMcfg9hrQw60waW1qjrd~pt2NlU8H79RMDTOYibN-SO3~cd~ptj7x4HBU6Oq3764Q1QYv04ocXSHcbFTFy5QuYwNHFGKnUgZf16Orik3I1UnJUSkXkYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Coimmunoprecipitation of p27Kip1 and cdk2. 107 U937 cells were treated with PMA for 48 hours. p27Kip1 was immunoprecipitated (IP-1, IP-2, and IP-3) three times from the same extract. Both the immunoprecipitate and the supernatant either before (Pre-IP supernatant [sup]) or after the immunoprecipitate (Post-IP sup) were run on an SDS polyacrylamide gel and Western blotted with p27 antibody. The Western blot was then stripped and reprobed with cdk2 antibody.
