Fig. 2.
![Fig. 2. Immunoblot analysis of HTLV-II virus lysates after reaction with MoAbs specific for HLA class-I (B9.12.1) or class-II (D1.12 and Tu35). Pellets of purified HTLV-II virions generated from C344 cell line (T) or from BJAB cells (B) were incubated in lysis buffer containing 0.2% sodium dodecyl sulfate (SDS) and equivalent concentrations of extracts, as shown by protein staining, analyzed by immunoblot in a 10% polyacrylamide gel containing 0.1% SDS. Immunoreactivity was visualized by chemiluminescence detection system. The size (in kilodaltons [k], indicated on the right) were determined by comparison with protein markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2296/3/blod4073202.jpeg?Expires=1735545723&Signature=t~ZalRCXmVSAmiPJmsG~JvL31nKdqHJ0iBOoSuHpOUgUeQJqfzjMm6EMubFtjo9r7l2JcllF3axMEXGsFR73wbOoSJQ6ePAML8Ke6j4ojzg6LY5LPV~xCelAIRvj329o2LfYyBoL8JB-7qvtFeQdw0VpcWFSUAmnSuQOuKVuurwST3rxyu7Bd95jBTf54qC5QkXl0myJDadQu80E-nEhz5SDJ4TSQXLeXyTl50KQtoPLHoH5tgjZwnuKZHOkvkPvjkEZP4epR7-ALY8VOUf9iWDQTYIH1OCEHzwlWOglyhmgew8AWOQQJnNPC5BOU9UfcLbl~w~N2a0BPUU02KHPdg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoblot analysis of HTLV-II virus lysates after reaction with MoAbs specific for HLA class-I (B9.12.1) or class-II (D1.12 and Tu35). Pellets of purified HTLV-II virions generated from C344 cell line (T) or from BJAB cells (B) were incubated in lysis buffer containing 0.2% sodium dodecyl sulfate (SDS) and equivalent concentrations of extracts, as shown by protein staining, analyzed by immunoblot in a 10% polyacrylamide gel containing 0.1% SDS. Immunoreactivity was visualized by chemiluminescence detection system. The size (in kilodaltons [k], indicated on the right) were determined by comparison with protein markers.
