Southern blot analysis of RT-PCR amplification products using FcγRII-specific primers. RNA isolated from the indicated cell sources was used to obtain cDNAs by reverse transcription. PCR amplification was performed using sets of primers specific for all FcγRII isoforms (A), Fcγ RIIa, c isoforms (B), and FcγRIIb isoforms (C). A 2% agarose gel was run and Southern blotting was performed as described in Materials and Methods with RS91-46 as the hybridizing probe. Specific bands of expected size for each primer pair were detected in each panel (334 bp for RS9145-RS9146 primer pair, 442 bp for RS9145-FcγIIA primer pair, and 441 bp/384 bp for RS9145-FcγIIB primer pair).