Fig. 5.
Cytokines and chemoattractants stimulate phosphorylation of PKB in human eosinophils. Phosphorylation was measured by an SDS-PAGE mobility shift as described in Materials and Methods. (A) Eosinophils (2 × 106) were stimulated with the cytokines IL-5 (10−9 mol/L), and IL-4 (10−8 mol/L) at 37°C for the indicated time intervals. (B) Eosinophils (2 × 106) were stimulated with the chemoattractants C5a (10−8 mol/L), PAF (10−6 mol/L), and RANTES (10−6) for 2 minutes at 37°C. (C) Eosinophils (2 × 106) were treated with IL-5 (10−9 mol/L) and PAF (10−6 mol/L) at 37°C for the indicated time intervals.
