The expression of cyclin E gene products. Equal amounts of whole cell lysates (10 μg) from AML blasts were applied in each lane. Full-length cyclin E is defined as the band of approximately 50 kD. Lanes number 1 to 4 correspond to the patient number in Table 2. The other 6 samples (lanes number 14 to 19) represent cyclin E⁻ AML blasts. “N” represents the lysate from mononuclear cells in normal BM. “CD34” and “Mono” represents the lysate from enriched CD34⁺ cells and monocytic cells separated from normal BM by the method as described in Materials and Methods. “U937” represents the lysate from U937 cells. The blots were reprobed with anti-actin antibody to show that each sample was equally loaded.