The expression of p27 gene product. Western blotting probed by anti-p27 antibody was performed. Equal amounts of whole cell lysates (10 μg) from AML samples with enhanced cyclin E expression (upper panel) and those without enhanced cyclin E (lower panel) were analyzed. Each number over the lane corresponds to the patient number in Table 2. “N” and “U937” represent the lysates from mononuclear cells in normal BM and U937 cells.