American Society of Hematology

Fig. 1.

$Fig. 1. Infection with HIV produces enhanced and novel patterns of phosphotyrosine-containing proteins. Activated peripheral blood lymphocytes (PBL) (lane 1), resting PBL (lane 2), and resting PBL from three patients with asymptomatic HIV infection (lanes 3 through 5) were lysed in RIPA lysis buffer, the total protein measured (Pierce), and 10 μg of total protein loaded onto each lane prior to fractionation by SDS-PAGE. The separated proteins were electrotransferred to nitrocellulose membranes and expression of phosphotyrosine (ptyr)-containing proteins analyzed by Western immunoblot. (A) Blots were probed with mouse anti-ptyr followed by goat-antimouse IgG conjugated to horseradish peroxidase. Blots were developed by enhanced chemiluminescence. Molecular mass standards are indicated on the left. (B) The blot was stripped and reprobed with anti-CD45 to confirm equal loading.$

Infection with HIV produces enhanced and novel patterns of phosphotyrosine-containing proteins. Activated peripheral blood lymphocytes (PBL) (lane 1), resting PBL (lane 2), and resting PBL from three patients with asymptomatic HIV infection (lanes 3 through 5) were lysed in RIPA lysis buffer, the total protein measured (Pierce), and 10 μg of total protein loaded onto each lane prior to fractionation by SDS-PAGE. The separated proteins were electrotransferred to nitrocellulose membranes and expression of phosphotyrosine (ptyr)-containing proteins analyzed by Western immunoblot. (A) Blots were probed with mouse anti-ptyr followed by goat-antimouse IgG conjugated to horseradish peroxidase. Blots were developed by enhanced chemiluminescence. Molecular mass standards are indicated on the left. (B) The blot was stripped and reprobed with anti-CD45 to confirm equal loading.

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