Peripheral blood lymphocytes enriched for CD8⁺ cells show increased Fyn specific kinase activity. The relative Fyn specific kinase activity was determined as described in Fig 3 from PBMC after depletion of CD4⁺ cells by two rounds of anti-CD4 magnetic beads. (A) The calculated Fyn-specific kinase activity from one each asymptomatic (Asx) HIV patient, AIDS patient, and normal, HIV seronegative control (Ctrl). (B) The Kinase assay and Western blot obtained for each of the individuals in (A) compared to a human T-cell line, Jurkat. The samples were run on the same gel, transferred to Immobilon-P, the kinase result visualized by autoradiography, and then the same blot probed with monoclonal anti-Fyn by Western immunoblotting and the results visualized by ECL. The lanes were cut from the original to align properly beneath each designated sample shown in (A).