Fig. 9.
DCs generated from CD34 depleted and unabsorbed cells are as potent as AC-derived DCs in stimulating allogenic T-cell proliferation. Graded numbers of DCs obtained after 7 days of culture in the presence of GM-CSF and IL-4 were used as stimulator cells for 1.5 × 105 allogenic purified T cells in 5-day cultures. Cell proliferation was assayed after a 12-hour pulse with 3H-TdR. Data are expressed as mean ± SD of sextuplet cultures. 3H-TdR incorporation of irradiated (30 Gy) DCs or purified T cells were less than 800 cpm. Results are representative of three experiments using cells from three different patients.
