Fig. 6.
![Fig. 6. Inhibition of VEGF expression by a catalytically inactive mutant Akt [HA-Akt(K179M)] in Ha-ras–transformed cells (NIH3T3R). NIH3T3R cells were cotransfected with HA-Akt(K179M) (5 μg) plasmid and a constant amount of reporter plasmid (2.5 μg; 1,511 bp; 385 bp; HIF-1 wt), and then exposed to hypoxia before assaying for luciferase activity. Values represent the means of at least three independent transfections. Error bars represent 1 SD of the mean.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3322/4/bl_0055f6.jpeg?Expires=1735562461&Signature=mHRmOiw~ZQPIrX0QKToPkbCkeW5uu2qyaylNZL9ybTEMk3Sg7v1GSbekjbQuc7KBETESaur9gqavDCJXZ0yNdWuaa-LUxxJFZw6p9eTmnkvc3eJrCiWkj2s~1nlWlckDTO0ZdY06JvB8GdCSKGNKWvfn8xK3yDmymwRxzKgdYpWYZqk~~jAJ~BaS~HRWxRH9A46KqiBYc2EKZtV84nJJrvt6f5gXrMFf0Xde~~nMzJLiNYRiKuOudp0Mvg6O~5e8eMiwAyUaQg6ox47vXPk2ZqNrQuUGT6ayNOeCz~IrPJmAwOm-dUZCiGw4BURsHv4ikLCQ8bG3bxbrkYeLZ~0axQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of VEGF expression by a catalytically inactive mutant Akt [HA-Akt(K179M)] in Ha-ras–transformed cells (NIH3T3R). NIH3T3R cells were cotransfected with HA-Akt(K179M) (5 μg) plasmid and a constant amount of reporter plasmid (2.5 μg; 1,511 bp; 385 bp; HIF-1 wt), and then exposed to hypoxia before assaying for luciferase activity. Values represent the means of at least three independent transfections. Error bars represent 1 SD of the mean.
