Induction of the non–NF-κB–dependent gene junB is not inhibited by expression of A20. PAEC were infected and stimulated as in (A) with TNF (T) and LPS (L), and RNA was extracted. Steady-state mRNA levels of A20, junB, and GAPDH were evaluated by Northern blot analysis as described in (A) using a junB cDNA probe shown to cross-react with its porcine homologue. Results show that the proto-oncogene junB is inducible in rAd.A20-infected PAEC, following TNF and LPS stimulation. The level of induction was even greater than that seen in the rAd.β-gal–infected PAEC, although this difference was not significant when corrected for GAPDH.