Flow cytometric record of the respiratory burst of neutrophils (10,000 cells counted) interacting with IC-bearing endothelial cells. Forward scatter (FSC), as a measure of the cell size, is presented on the x-axis, whereas side scatter (SSC), as a measure of granularity, is presented on the y-axis. The elliptic gate depicts neutrophils. The difference between cell populations in FSC was used to establish gates for the analysis of the dihydrorhodamine-123 oxidation in neutrophils.