Fig. 2. Induction of TGase II mRNA in... | American Society of Hematology

Fig. 2.

$Fig. 2. Induction of TGase II mRNA in RPMI 8226 cells. Cells were treated or not for 7 hours with 10−7 mol/L atRA in defined medium. Total RNA (1 μg) was submitted to RT and PCR (for 22, 28, 34, or 40 cycles), fractionated on 2% agarose gel, transferred to a Hybond N+ membrane, and hybridized to32P-labeled oligonucleotidic probes for human TGase II and actin. (For primers and probes see Materials and Methods).$

Induction of TGase II mRNA in RPMI 8226 cells. Cells were treated or not for 7 hours with 10⁻⁷ mol/L atRA in defined medium. Total RNA (1 μg) was submitted to RT and PCR (for 22, 28, 34, or 40 cycles), fractionated on 2% agarose gel, transferred to a Hybond N⁺ membrane, and hybridized to³²P-labeled oligonucleotidic probes for human TGase II and actin. (For primers and probes see Materials and Methods).

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