Lck is functional in CLL B cells. Quantitation of³²P labeling of enolase by Lck in in vitro kinase assays of lysates of either normal peripheral blood B cells or B cells from three representative B-CLL patients (B-CLL), a hairy cell leukemia (HCL) patient, a patient with non-Hodgkin's lymphoma (NHL) in the leukemic phase, or normal T cells. Equal amounts of proteins for each lysate were used for Lck immunoprecipitation. An anti-Lck immunoblot of the same samples is shown below the graph. Each kinase assay was repeated at least twice.