Galectin-3–induced activation of the NADPH-oxidase in exudated neutrophils. The NADPH-oxidase activity in peripheral blood neutrophils (106 cells; dashed lines) or exudated neutrophils (106 cells; solid lines) was assessed. The figure shows the neutrophil responses to galectin-3 (20 μg/mL; a and d), galectin-3 (20 μg/mL) in the presence of lactose (10 mmol/L; b and e), and collagenase digested galectin-3 (CRD; 20 μg/mL; c and f). The extracellular release of superoxide anion (a through c) was measured in the presence of HRP (4 U) and isoluminol (5 × 10−5 mol/L) while the intracellular production of superoxide (d through f) was measured in the presence of SOD (50 U), catalase (2,000 U), and luminol (5 × 10−5 mol/L). Responses are given as chemiluminescence (CL) units in megacounts per minute (Mcpm).
