American Society of Hematology

Fig. 9.

$Fig. 9. A Western blot with separated proteins from neutrophil plasma membrane (γ2) and cytosol, corresponding to the fractionated content of 5 × 106 and 1 × 106 cells, respectively. The blot was incubated with antibodies directed against galectin-3 (anti–Mac-2 antibodies; culture supernatant from the hybridoma M3/38; 1/25) followed by HRP-labeled rabbit anti-rat Ig antibodies (DAKO P0450; 1/1,000), after which the blot was developed with peroxidase substrate (VIP Kit; Vector). Molecular sizes are given in kilodaltons.$

A Western blot with separated proteins from neutrophil plasma membrane (γ2) and cytosol, corresponding to the fractionated content of 5 × 10⁶ and 1 × 10⁶ cells, respectively. The blot was incubated with antibodies directed against galectin-3 (anti–Mac-2 antibodies; culture supernatant from the hybridoma M3/38; 1/25) followed by HRP-labeled rabbit anti-rat Ig antibodies (DAKO P0450; 1/1,000), after which the blot was developed with peroxidase substrate (VIP Kit; Vector). Molecular sizes are given in kilodaltons.

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