Involvement of FN-integrin interaction in augmentation of clonogenic growth of human hematopoietic progenitor cells. The human CD34+ cells were incubated with 100 μg/mL of BSA or FN in serum-free IMDM at 37°C for 1 hour in the presence or absence of the indicated peptides (10 μg/mL) and subsequently cultured in methylcellulose media at 5.0 × 102/mL to assess their clonogenic growth. GRGESP and β3 721-744 were used as control peptides for GRGDSP and CS1, respectively. The reduction in colony numbers was used to calculate the percentage inhibition relative to the FN-increased colony number. The data represent mean percent inhibition ± SD of triplicate cultures. Statistically significant differences from respective control values are indicated by one (P < .05) or two (P < .01) asterisks. Similar results were obtained in three independent experiments and when the peptides were used at 100 μg/mL.
