Fig. 6.
Fig. 6. Methylation pattern of the Hif1a exon I.2 region. (A) Map of the CpG methylation-sensitive restriction enzyme recognition sites selected to assess the methylation pattern of distinct CpG dinucleotides in the exon I.2 region. The methylation status of a particular restriction enzyme site is indicated by open and partially filled circles. (B) Southern blot analysis of genomic DNA isolated from various mouse cell lines and tissues. The DNA was cleaved either withXbaI alone, or in combination with CpG methylation-sensitive restriction enzymes cutting 5′ (Cfr42I, C), within (SmaI, S), or 3′ (NotI, N) of exon I.2. (C) Detailed exon I.2 upstream Southern blot analysis using the CpG methylation-sensitive restriction enzymes Cfr42I (C),HhaI (H) and HpaII (P), or the methylation-insensitiveHpaII-isoschizomer MspI (M). Note that anHpaII/MspI restriction fragment length polymorphism (RFLP) was detected.

Methylation pattern of the Hif1a exon I.2 region. (A) Map of the CpG methylation-sensitive restriction enzyme recognition sites selected to assess the methylation pattern of distinct CpG dinucleotides in the exon I.2 region. The methylation status of a particular restriction enzyme site is indicated by open and partially filled circles. (B) Southern blot analysis of genomic DNA isolated from various mouse cell lines and tissues. The DNA was cleaved either withXbaI alone, or in combination with CpG methylation-sensitive restriction enzymes cutting 5′ (Cfr42I, C), within (SmaI, S), or 3′ (NotI, N) of exon I.2. (C) Detailed exon I.2 upstream Southern blot analysis using the CpG methylation-sensitive restriction enzymes Cfr42I (C),HhaI (H) and HpaII (P), or the methylation-insensitiveHpaII-isoschizomer MspI (M). Note that anHpaII/MspI restriction fragment length polymorphism (RFLP) was detected.

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