Representative D-FISH patterns in interphase nuclei and metaphase cells. (A) Normal, 2R2G. (B) One copy of t(9;22)(q34;q11.2), 1R1G2F. (C) t(9;22)(q34;q11.2), 1R1G2F. (D) One copy of t(9;22)(q34;q11.2), 2R2G1F caused by a split BCR and ABL signal on abnormal chromosome 9. (E) One copy of t(9;22)(q34;q11.2) and an additional Ph chromosome, 1R1G3F. (F) t(9;2210)(q34;q11.2;q22), 2R2G1F. (G) Atypical pattern 1R1G1F, t(9;22)(q34;q11.2). (H) Atypical pattern 2R1G1F, t(9;22)(q34;q11.2). (I) 1R2G1F, metaphase with a masked Ph chromosome caused by insertion translocation of ABL into BCR. (J) Atypical pattern 1R2G1F, t(9;22)(q34;q11.2). (K) Nucleus with a false-positive pattern due to signal separation and coincidental overlap of BCR and ABL signals. (L) 1R1G1F, metaphase with no BCR or ABL on the abnormal chromosome 9 (arrow).