LPS desensitization diminishes TNF-α and IL-10 production by human DC. DC were cultured for 5 days in the absence or presence of LPS at the concentrations indicated (primary culture). Day-5 DC were recultured for 48 hours with or without LPS (10 ng/mL) and supernatants were analyzed for (A) TNF-α, (B) IL-10, and (C) IL-8 levels using specific ELISAs. Data represent mean values of triplicate measurements with standard deviation (SD) from one experiment representative of eight independent experiments.