Fig. 3.
Factor XIIIa–catalyzed cross-linking of the fibrin γ-chain analyzed by immunoblotting. Fibrinogen was clotted with thrombin and factor XIII in the presence of CaCl2. At timed intervals, the clots were subjected to SDS-PAGE followed by immunoblotting using an anti–γ-chain antibody. Besides the γ-dimer, high-molecular-weight polypeptides 1 and 2 were formed in the Marburg fibrin, and the corresponding peptides, N1 and N2, were formed in the normal fibrin. The molecular mass and the positions of the marker proteins are indicated in the left margin.
