Fig. 5.
Fig. 5. Factor XIIIa–catalyzed cross-linking of α2-PI to the Marburg fibrin analyzed by autoradiography. Fibrinogen was clotted with thrombin and factor XIII in the presence of125I–α2-PI and CaCl2 at 37°C for 30 minutes. After extensive washing, the radiolabeled clots were subjected to SDS-PAGE followed by autoradiography. Band 1 represents α2-PI and bands 2 and 3 represent a 115-kD (Marburg α-chain–α2-PI) and a 135-kD (normal α-chain–α2-PI) complex, respectively. Bands 4 and 5 represent high-molecular-weight complexes.

Factor XIIIa–catalyzed cross-linking of α2-PI to the Marburg fibrin analyzed by autoradiography. Fibrinogen was clotted with thrombin and factor XIII in the presence of125I–α2-PI and CaCl2 at 37°C for 30 minutes. After extensive washing, the radiolabeled clots were subjected to SDS-PAGE followed by autoradiography. Band 1 represents α2-PI and bands 2 and 3 represent a 115-kD (Marburg α-chain–α2-PI) and a 135-kD (normal α-chain–α2-PI) complex, respectively. Bands 4 and 5 represent high-molecular-weight complexes.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal