Localization of CD30+ and IL-4R+ cells in the medullary areas of postnatal thymus. (A) Autoradiograph of a thymus cryostat section hybridized with 35S-labeled antisense CD30 probe, showing positive signal in the medullary areas and along the septa (dark field, original magnification ×40). (B) Autoradiograph of a consecutive section hybridized with sense CD30 probe, showing virtually no signal (dark field, original magnification ×40). (C) Autoradiograph of a thymic medullary area hybridized with antisense CD30 probe showing high CD30 mRNA expression (dark field, original magnification × 100). (D) Autoradiograph of a consecutive section hybridized with sense CD30 probe showing no signal (dark field, original magnification ×100). (E) CD30 immunoreactive cells in the thymic medulla. Section was immunostained with anti-CD30 MoAb, using the avidin-biotin-peroxidase method, and the AEC substrate (red color, original magnification ×100). (F) IL-4R immunoreactive cells in the thymic medulla (red color, original magnification ×100).
